Composite
Ag43+FLAG

Part:BBa_K1352001:Design

Designed by: Martyna Sroka, Ana-Maria Cujba   Group: iGEM14_Aberdeen_Scotland   (2014-10-02)


Ag43+FLAG+MCS under the control of pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1678
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1981
    Illegal NgoMIV site found at 3205
    Illegal NgoMIV site found at 3715
    Illegal NgoMIV site found at 3736
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2731
    Illegal AgeI site found at 3475
    Illegal AgeI site found at 3889
    Illegal AgeI site found at 4131
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

FLAG tag epitope flanked by multiple cloning sites was designed to be in-frame with Ag43 ORF; it is optimized for E.coli codon bias.


Source

This BioBrick was PCR amplified from Bba_K1352000; in-frame FLAG tag epitope flanked by multiple cloning sites was inserted via In-Fusion (Clonetech).

References